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Environmental Analytical Methods (1599), 2013/14

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Environmental Analytical Methods
UMH subject: 1599




Miguel Ángel Sogorb
Jorge Estévez

Dept. Applied Biology

Area Toxicology

This subject has been teached in 
Degree in Environmental Science

Course Description

  • To list and describe the stages of a quantitative chemical analysis.
  • To list and describe the general principles of the sampling methodology.
  • To calculate the residual concentration of an analyte after a liquid-liquid extraction in certain conditions.
  • To explain the basis for microwave digestion and sonication.
  • To explain the differences among by dry, wet and proteolytic digestions.
  • To explain the operation of a Soxhlet extractor.
  • To explaining at least one technique deproteinization of biological tissues.
  • To recognize the basic components of a supercritical fluid extractor.
  • To explain which bases its efficiency technique supercritical fluid extraction.
  • To differentiate between different modes of supercritical extraction.
  • To explain the differences between the different modes of solid-liquid extraction.
  • To name at least 3 different types of stationary phases used in solid-liquid extraction.
  • To describe the physical and chemical fundamentals of the interactions (polar, non-polar and ionic) that occur between analytes and stationary phases in solid-liquid extractions with minicolumns.
  • To define the terms solvation, retention, elution, capacity and selectivity (based on solid-liquid extraction with minicolumns).
  • To predict how a change of solvent conditions to the retention or elution of an analyte in a liquid-solid extraction minicolumns.
  • To describe the stages of a solid phase microextraction.
  • To describe the stages of a solid phase micro magnetic bar.
  • To qualitatively describe the areas of the electromagnetic radiation spectrum.
  • To establish appropriate relationships between wavelength, frequency and energy of an electromagnetic wave.
  • To define the concepts of transmittance and absorbance.
  • To solve problems by applying the Lambert-Beer law.
  • To describe the parts of a spectrophotometer.
  • To explain the differences between absorption spectroscopy, and fluorescence emission.
  • To draw a diagram of a molecular absorption spectrophotometer, a fluorometer, and atomic emission spectrometry.
  • To explaining the operation of sources of visible, ultraviolet and infrared spectroscopy most common.
  • To describe the operation of a concentric type nebulizer and of at least two different types of atomizers used in atomic spectroscopy.
  • To describe the operation of a hollow cathode lamp.
  • To describe the operation of a plasma torch.
  • To explain the basis for the determination of mercury by cold vapor technique.
  • To explain the advantages of the hydride generation system for the determination of Pb, As, Ge and Bi.
  • To define the concepts of stationary phase support, eluent and mobile phase.
  • To calculate for a given chromatogram following chromatographic parameters: retention volume, number of theoretical plates equivalent to theoretical plate height, capacity factor, separation factor and resolution.
  • To establish a classification of chromatographic techniques based on the arrangement of the stationary phase and the physical state of the mobile phase.
  • To describe the basic principles of chromatography separation of analytes by normal phase adsorption, reverse-phase adsorption, distribution, ion exchange, gel filtration and affinity.
  • To explain the principles of operation of the thin layer chromatography.
  • To define the Rf factor in thin layer chromatography.
  • To predic Rf factor as affect a given change in the polarity of the solvent in a separation thin layer chromatography.
  • To describe the basic components of a system of high-performance liquid chromatography.
  • To explainin the operation of the following liquid chromatography detectors such as fluorescence, conductivity , amperometric and UV-visible diode array.
  • To describe the basic components of a gas chromatography system.
  • To describe the types of common stationary phase in gas chromatography.
  • To describe the differences between the injection modes "with" and "without" flow division in gas chromatographic system.
  • To explain the influence of temperature on the gas chromatographic separations.
  • To explain the operation of the following gas chromatography detectors: flame ionization, electron capture, nitrogen and phosphorus thermal conductivity.
  • To describe the basic components of a capillary electrophoresis system.
  • To define the following concepts: electroosmotic flow, electrical mobility and effective mobility on a capillary electrophoresis system.
  • To explain how works most common detectors in capillary electrophoresis.
  • To describe the basic operating principles of capillary electrophoresis micellar free zone, and isoelectric focusing gel.
  • To describe the basic principles that govern the gravimetric and volumetric methods.
  • To describe the basis of a volumetric method of neutralization, precipitation and complex formation.
  • To enumerate least three different analytes analyzable by each of the following techniques: 1) an assessment by neutralization , 2 ) precipitation titration , and 3 ) assessment for complex formation.
  • To describe the general principles of gravimetric methods of precipitation and volatilization.
  • To describe the basic parts of a mass spectrometer .
  • To explain the operation of these systems: ionization impact, electronic assisted laser desorption ionization, atmospheric pressure chemical and atmospheric pressure ionization electrospray.
  • To explain how the following mass spectrometry analyzers: magnetic sector double sector and quadrupole time of flight.
  • To explain the operation of a mass spectrometry detector.
  • To describe the differences between SIM detection mode and SCAN mode.
  • To explain what a tandem mass spectrometer.
  • To describe the advantages of a detector mass against another type of detector coupled to a gas chromatograph.
  • To write the advantages of a detector mass against another type of detector coupled to a liquid chromatograph.
  • To describe the advantages of a system coupled to mass spectrometry with inductively coupled plasma (ICP) compared to other systems of nuclear detection and quantification.
  • To list several applications of mass spectrometry coupled to inductively coupled plasma.
  • To perform the calculations necessary to prepare solutions of the required concentrations.
  • To pipet skill.
  • To calculate, having suitable patterns, the problem of an analyte concentration using colorimetric and chromatographic analytical techniques.
  • To perform appropriate procedures for determining the alkalinity of a water sample by an acid-base titration.
  • To separate two analytes using a technique of solid-liquid extraction.
  • To use, following the teacher's instructions, the following equipment: 1) Soxhlet extraction equipment, 2) liquid chromatograph high resolution, 3) a gas chromatograph; 4) an induction-coupled plasma mass spectrometer.


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Copyright 2012, Universidad Miguel Hernandez. Reconocer autoría/Citar obra. Sánchez, M. Á. S., Domenech, J. E. (2012, June 11). Environmental Analytical Methods (1599). Retrieved January 20, 2022, from OCW - Universidad Miguel Hernández de Elche Web site: http://ocw.umh.es/ciencias/environmental_analytical_methods%20. Esta obra se publica bajo una licencia Licencia Creative Commons Licencia Creative Commons
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